Commission Implementing Regulation (EU) No 399/2014 of 22 April 2014 concerning the authorisation of the preparations of Lactobacillus brevis DSM 23231, Lactobacillus brevis DSMZ 16680, Lactobacillus plantarum CECT 4528 and Lactobacillus fermentum NCIMB 30169 as feed additives for all animal species (Text with EEA relevance)

Article 1 Authorisation

The preparations specified in the Annex belonging to the additive category ‘technological additives’ and to the functional group ‘silage additives’, are authorised as additives in animal nutrition, subject to the conditions laid down in that Annex.

Article 2 Transitional measures

The preparations specified in the Annex and feed containing them, which are produced and labelled before 13 November 2014 in accordance with the rules applicable before 15 May 2014 may continue to be placed on the market and used until the existing stocks are exhausted.

Article 3 Entry into force

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.

This Regulation shall be binding in its entirety and directly applicable in all Member States.

ANNEX

Identification number of the additive	Name of the holder of authorisation	Additive	Composition, chemical formula, description, analytical method	Species or category of animal	Maximum age	Minimum content	Maximum content	Other provisions	End of period of authorisation
Identification number of the additive	Name of the holder of authorisation	Additive		Species or category of animal	Maximum age	CFU/kg of fresh material		Other provisions	End of period of authorisation
Category of technological additives. Functional group: silage additives.
1k20744	—		Additive composition Preparation of Lactobacillus brevis DSM 23231 containing a minimum of 1 × 10¹⁰ CFU/g additive. Characterisation of the active substance Viable cells of Lactobacillus brevis DSM 23231. Analytical method⁽¹⁾ Enumeration in the feed additive: spread plate methodusing MRS agar (EN 15787). Identification: Pulsed Field Gel Electrophoresis (PFGE).	All animal species	—	—	—	1. In the directions for use of the additive and premixture, indicate the storage conditions. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 5 × 10⁷ CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection, eye protection and gloves during handling.	13 May 2024
1k20745	—	Lactobacillus brevis DSMZ 16680	Additive composition Preparation of Lactobacillus brevis DSMZ 16680 containing a minimum of 2,5 × 10¹⁰ CFU/g additive. Characterisation of the active substance Viable cells of Lactobacillus brevis DSMZ 16680. Analytical method⁽¹⁾ Enumeration in the feed additive: spread plate method using MRS agar (EN 15787). Identification: Pulsed Field Gel Electrophoresis (PFGE).	All animal species				1. In the directions for use of the additive and premixture, indicate the storage conditions. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10⁸ CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection, eye protection and gloves during handling.	13 May 2024
1k20746	—	Lactobacillus plantarum CECT 4528	Additive composition Preparation of Lactobacillus plantarum CECT 4528 containing a minimum of 2,5 × 10¹¹ CFU/g additive. Characterisation of the active substance Viable cells of Lactobacillus plantarum CECT 4528. Analytical method⁽¹⁾ Enumeration in the feed additive: spread plate method using MRS agar (EN 15787). Identification: Pulsed Field Gel Electrophoresis (PFGE).	All animal species	—	—	—	1. In the directions for use of the additive and premixture, indicate the storage conditions. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10⁹ CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection, eye protection and gloves during handling.	13 May 2024
1k20747	—		Additive composition Preparation of Lactobacillus fermentum NCIMB 30169 containing a minimum of 2,5 × 10¹⁰ CFU/g additive. Characterisation of the active substance Viable cells of Lactobacillus fermentum NCIMB 30169. Analytical method⁽¹⁾ Enumeration in the feed additive: spread plate method using MRS agar (EN 15787). Identification: Pulsed Field Gel Electrophoresis (PFGE).	All animal species	—	—	—	1. In the directions for use of the additive and premixture, indicate the storage conditions. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10⁸ CFU/kg fresh. 3. For safety: it is recommended to use breathing protection, eye protection and gloves during handling.	13 May 2024